Figure 6—figure supplement 5.

Figure 6—figure supplement 5. CRISPR/Cas9-mediated mutagenesis of zswim4-6 results in the expansion of chordin expression in mosaic mutants (F0).

(A) Bilateral expression of chordin in wild-type 2d planula and corresponding sequencing chromatogram of zswim4-6_g254 target region. (B–O) Expanded or radialized chordin expression in 2d mutant planulae injected with a single guide RNA (B–I) or two guide RNAs (J–O) targeting the SWIM zinc-finger domain. Corresponding sequencing chromatograms show sequence variability (overlapping peaks, gray or no letters) in the vicinity of the targeting locus of the zswim4-6_g254 gRNA in individual mutant embryos. The protospacer sequence is underlined, the position of the PAM is shown on (A). Scissors indicate the Cas9 cutting site. In the in situ hybridization images, asterisks mark the oral side, scale bars 100 µm.