Figure 6—figure supplement 3—source data 1.

Figure 6—figure supplement 3—source data 1. ZSWIM4-6 knockdown partially rescues the reduction of pSMAD1/5 caused by GDF5-like knockdown.

Oral views of 2 dpf embryos stained for pSMAD1/5. (A, B) Embryos injected with control morpholino and ZSWIM4-6MO show phenotypes identical to the ones presented in Figure 6C and C’. (C, D) Embryos injected with GDF5lMO together with either Control MO (C) or ZSWIM4-6MO (D). pSMAD1/5 is strongly reduced upon GDF5lMO injection (C), but partially rescued by co-injection of ZSWIM4-6MO (D). (E, F) Quantification of the ectodermal (E) and the endoderm (F) nuclear pSMAD1/5 staining intensity in the GDF5lMO morphants. Nuclear staining intensity (arbitrary units, a.u.) is plotted as a function of the relative position of each nucleus in the endoderm or in the ectoderm along a 180° arc from 0 (high signaling side) to π (low signaling side). The measurements from GDF5lMO + ControlMO embryos (n = 9) and GDF5lMO + ZSWIM4-6MO embryos (n = 16) are described by a LOESS smoothed curve (solid line) with a 99% confidence interval for the mean (shade).

Figure 6—figure supplement 3—source data 1.