Figure 6—figure supplement 2—source data 1.

Figure 6—figure supplement 2—source data 1. zzswim5 overexpression dampens BMP signaling and causes developmental defects in zebrafish.

(A–F”) Wild-type zebrafish embryos were left uninjected (B) or injected at the one-cell stage with 20, 40, 80, or 210 pg zzswim5 mRNA. (A) Phenotype quantification at 1 d post-fertilization (dpf) shows increasingly severe developmental defects at higher amounts of mRNA. A representative selection was imaged at 1 dpf (B–F’’). Multiple embryos are shown for 80 (E–E”) and 210 (F–F”) pg to illustrate the variety of defects. Number of embryos – uninjected: 19, 20 pg: 20, 40 pg: 24, 80 pg: 21, 210 pg: 17. (G–I) Embryos were injected with 80 pg zzswim5 mRNA or left uninjected and fixed at 50% epiboly (early gastrulation). BMP signaling levels were assessed using pSmad1/5/9 immunostaining. Animal pole views are shown with ventral on the left. (G) Quantification of immunofluorescence (arbitrary units, a.u.) reveals lower amplitude BMP signaling gradients in zzswim5-overexpressing embryos (I) compared to uninjected controls (H). The measurements from uninjected embryos (n = 8) and zzswim5-injected embryos (n = 8) are described by a LOESS smoothed curve (solid line) with a 99% confidence interval for the mean (shade).

Figure 6—figure supplement 2—source data 1.