Figure 5—figure supplement 1—source data 1.

Figure 5—figure supplement 1—source data 1. zswim4-6 reacts differently to the downregulation of BMP2/4 and GDF5-like. (A) Expression domains and putative interactions of the BMP network components in 2d embryos (based on Genikhovich et al., 2015). (B–F’) Effect of the knockdown (KD) of the individual BMP signaling components on nuclear pSMAD1/5 and on zswim4-6 expression. zswim4-6 expression is abolished upon KD of bmp2/4 and chordin, but not in KD of gdf5-l and gremlin. Sketches in (B–F) show that the gradient is lost upon BMP2/4 and Chd KD, reduced upon GDF5-like KD and expanded upon Gremlin KD (based on Genikhovich et al., 2015). (G–L) KD of bmp2/4 using different concentrations of BMP2/4MO results in either normal, bilaterally symmetric marker expression or complete radialization but no intermediate phenotypes. Instead, the penetrance of the radialization phenotype increases with the increase of the BMP2/4MO concentration. Co-injection of the lowest, ineffective BMP2/4MO concentration with GDF5lMO also results in a complete radialization. All stainings are performed on 2d planulae. Scale bars 100 µm.