Fig. 7 Tyrosine hydroxylase (TH) expression was evaluated in the parvocellular pretectal nucleus (PPN) 30 days after spontaneous nicotine withdrawal in zebrafish. Top: representative images obtained from coronal sections through the PPN show TH immunofluorescence in (a) control (drug-free water + water), (b) nicotine + water, (c) water + fluoxetine and (d) nicotine + fluoxetine. (e) quantitative analysis is expressed as a percentage of the mean area control value. Data are mean ± SEM of the mean of 3/5 zebrafish/group. Each dot represents one animal. *p < 0.05 (one-way analysis of variance (ANOVA) followed by post hoc t test).
                
                    
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      Full text @ J. Psychopharmacol. (Oxford)