Figure 4.

Figure 4. Modulation of Tsc1 alters key host defence and inflammatory pathways.

(A) Heatmap of differentially expressed genes (DEGs) identified by RNA sequencing of 3 dpf embryos after CRISPR-Cas9 knockdown of tsc1a. (B) Enrichment score mapping to the term MTOR signalling pathway of DEG identified by RNA sequencing of 3 dpf embryos after CRISPR-Cas9 knockdown of tsc1a. (C) Heatmap of DEGs identified by RNA sequencing of 3 dpi embryos after CRISPR-Cas9 knockdown of tsc1a and infection with M. marinum. (D) Volcano plot of DEGs identified by RNA sequencing of 3 dpi embryos after CRISPR-Cas9 knockdown of tsc1a and infection with M. marinum. Up-regulated host defence and inflammatory pathway genes are annotated. (E, F) Gene ontology analysis was performed on DEGs identified by RNA sequencing of 3 dpi embryos after CRISPR-Cas9 knockdown of tsc1a and infection with M. marinum to identify key downstream pathways either increased (E) or decreased (F) in tsc1a crispants.