Figure 9—figure supplement 1.

Figure 9—figure supplement 1. Neutrophils remain motile during the establishment of Salmonella persistent infection.

(A, B) Tg(mpx:GFP) larvae were injected with PBS or Salmonella (Sal)-DsRed in HBV and time-lapse videos of labeled neutrophils were acquired during 1.5 hr every 1 min 30 s at 1 hpi or 4 dpi. (A) Quantification of the individual neutrophil velocity from PBS-injected or Sal-infected larvae at 1 hpi and 4 dpi. One replicate used for 1 hpi and data of two replicates pooled for 4 dpi (mean velocity/neutrophil ± SEM, at 1 hpi: n_PBS = 22, n_Sal = 44; at 4 dpi: n_PBS = 26, n_Sal = 63; Mann–Whitney test, two-tailed, significance of Sal versus PBS conditions, ****p < 0.0001, ns: not significant). (B) Migration of neutrophils in response to PBS or Sal-DsRed at 1 hpi or 4 dpi. Representative trajectory plots of individual neutrophil movement tracks are shown, with the initial position in the center of the graph. Number of neutrophil tracks are indicated.