Figure 8.
Salmonella persistence induces large clusters of pro-regenerative wt1b-expressing macrophages.
(A–D) Tg(mfap4:mCherry-F/wt1b:GFP-F) larvae were injected with PBS or Sal-E2Crimson in the HBV. (A) Representative maximum projections of fluorescent confocal images showing macrophages (mfap4+ cells, red) and wt1b-expressing cells (wt1b+ cells, green) after Salmonella infection at (A) 4 hpi and at (B) 4 dpi. Asterisk: auto-fluorescence. Scale bar: 30 μm. Big clusters of wt1b-expressing macrophages (mfap4+-wt1b+ cells, yellow) were observed at 4 dpi. (C, D) Zoom of two representative fluorescent confocal images showing large clusters of wt1b-expressing macrophages (mfap4+-wt1b+ cells, yellow) at 4 dpi. Scale bar: 10 μm, arrow: mfap4+-wt1b+ macrophage.
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