Figure 5.

Figure 5. Salmonella persistence leads to a higher proportion of macrophages containing bacteria.

Tg(mfap4:mCherry-F/tnfa:GFP-F) and Tg(mfap4:mCherry-F/mpx:GFP) larvae were injected with PBS or Sal-E2Crimson in HBV. (A) Quantification of the percentage of infected macrophages in Sal-infected larvae at indicated time post-infection. Data of three replicates pooled (mean percentage/larva ± SEM, n_{3–14 hpi} = 15 embryos and n_{4 dpi} = 43 embryos). (B) 3D reconstruction confirming the intramacrophagic and intraneutrophilic localization of a bacterial aggregate at 3 hpi and 4 dpi. Scale bar: 20 μm. (C) Size of intracellular E2Crimson⁺ events (in μm³), quantified following 3D reconstruction. Median volume, n_{5 hpi} = 506, n_{4 dpi} = 990, Mann–Whitney test, two-tailed, ***p < 0.001. (D) Size repartition of E2Crimson⁺ events (10 embryos were imaged at each time point).