Figure 2—figure supplement 1.

Figure 2—figure supplement 1. Expression of tnfa, cxcr4b, cxcl12a mRNA during Salmonella infection.

RT-qPCR analysis of (A) tnfa, (B) cxcr4b, and (C) cxcl12a mRNA expression. Embryos were either injected with PBS or Sal-GFP and larvae presenting a high bacterial proliferation were excluded from the analysis. RNA samples were extracted from whole larvae at 3 hpi, 1 dpi, 2 dpi, 3 dpi, and 4 dpi. Data are presented as relative target gene/ef1a expression in the Sal-infected or PBS-injected larvae using the formula 2^−ΔCp. Of note, as tnfa expression was usually not detected in PBS condition, data could not be presented as relative expression (Infected versus PBS) as for other genes. Values are the means ± SEM of eight replicates. Per replicate, eight larvae per time points were used (Mann–Whitney test, two-tailed **p < 0.01; ***p < 0.001, ns: not significant).