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Fig. 7 Inhibiting thyroid hormone signalling with TBBPA produced a global delay in development. (A) Gross images of representative larvae showed developmental delay over multiple timepoints (10 and 25 hours after treatment) for TBBPA- or NH-3-treated larvae, compared to untreated or DMSO-treated larvae. Although dorsal images are shown here to emphasize head size and shape, samples were correlated with and staged according to external criteria from the ventral and side profiles as well (e.g., number of intestinal coils, angle of proctodeum, etc). (B) Independent samples t-tests demonstrated that both TBBPA-treated and NH-3-treated larvae showed significantly delayed morphological developmental staging features, compared to untreated or DMSO-treated larvae. NH-3 data were from a single trial only. Asterisks indicate significance at *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. TBBPA, tetrabromobisphenol A