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Fig. 6 Ceratohyal hypertrophy and earlier mesenchymal condensation might have been delayed by the thyroid hormone antagonist TBBPA. (A-C) Safranin O staining revealed that ceratohyals of untreated (A), DMSO-treated (B), and TBBPA-treated (C) embryos had all undergone hypertrophy 30 hours after treatment. (D-F) Earlier, 26 hours after treatment, Safranin O staining suggested that ceratohyal chondrocytes of untreated (D) and DMSO-treated (E) were more hypertrophic than TBBPA-treated (F) embryos. (G-I, white arrows) Even earlier, Safranin O staining suggested that ceratohyals of untreated (G) and DMSO-treated (H) had undergone mesenchymal condensation 7 hours after treatment, but condensation of TBBPA-treated (I) mesenchyme appeared delayed. (J) Measurements of chondrocyte cross-sectional areas after 30 hours of treatment in untreated, DMSO-, and TBBPA-treated groups. Abbreviations: N, total number of cells measured from three biological replicates; n, number of cells from one sample. Asterisks indicate significance at *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 and error bars indicate a 95% confidence interval. Scale bars = 50 μm. TBBPA, tetrabromobisphenol A