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Figure 4
DA neuron isolation and fluorescence intensity analysis
Example steps in the CellProfiler pipeline that highlights the identification of TH neurons based on the pixel size.
(A) Using the 20x bright field image to overlay the fluorescent image to automatically detect the eyes for removal of autofluorescence.
(B) The “IdentifyPrimaryObjects” command with the global threshold strategy configurations correctly capture the neurons that are later used to quantify intensity.
Acknowledgments
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