ZFIN Image: ?tefl et al., 2024, Figure 1

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Figure Caption

Figure 1

Caveolar proteins do not accumulate at the plasma membrane lesion site in zebrafish embryos

(A) Caveolar proteins do not accumulate in the lesion patch. Fluorescence images of individual cells expressing Caveolin3-Clover, Clover-Cavin1a, EHD2a-Clover, and TinyDysf-Clover before and after laser-induced damage. The lesioning sites are marked by red arrows; white boxes surrounding the lesion sites mark regions with pronounced intensity changes. Scale bar (applies to all panels), 5 μm.

(B) Caveolar proteins show distinct subcellular distributions after membrane lesioning. Time-dependent fluorescence of caveolar proteins fused to Clover at the lesion site (red) and in the cytoplasm (blue), respectively. Thick lines show averages over multiple cells (cell numbers, N, are given in each panel); shaded regions indicate standard errors of the mean. Fluorescence signals were set to 100 at t = 0.

Acknowledgments

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