ZFIN Image: Nielson et al., 2023, Fig 3

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Figure Caption

Fig 3

Yeast were incubated overnight in the indicated conditions, then isolated and fixed for microscopy at 63×/1.4NA. MECs have increased exposure to mannose and chitin despite the production of a thick capsule. Concanavalin (A) and wheat germ agglutinin (B) mean fluorescence intensities for Cn cultured in YPD, TC-experienced, or J774-experienced. Statistical comparisons represent the results of two-tailed, unpaired t-tests. (C) India ink and fluorescence images of YPD-cultured, TC-experienced, and J774-experienced Cn, three examples each. Staining in the first two conditions is punctate, at bud scars, or absent, while staining of J774-experienced cells is both punctate and circumferential. ConA is tagged with Alexa Fluor 633 (Fisher #C21402). Widefield images were taken with a 20×, 0.85NA objective. Exposures and intensity adjustments are uniform for all panels. (D) Example of ConA and WGA staining that overlaps on a different J774-experienced cell. Note intense staining at the bud scar with overlapping circumferential staining. WGA is tagged with Alexa Fluor 555 (Fisher #W32464). Widefield images were taken with a 20×, 0.85NA objective.

Acknowledgments

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