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Fig. 6 CHNQD-00824-induced apoptosis and cell cycle arrest in BT549 cells. (A,C) Flow cytometry analysis of cell cycle analysis on BT549 cells after treatment with CHNQD-00824 for 24 h. (B,D) Flow cytometry analysis of BT549 cells stained with the Annexin V/FITC-PI double staining solution after treatment with CHNQD-00824 for 24 h. (E) Effect of CHNQD-00824 on apoptosis-related proteins. BT549 cells were treated with CHNQD-00824 (0–8 μM) for 24 h, then cells were harvested and proteins were separated by electrophoresis on SDS-PAGE. Western blotting analysis were performed to detect the expression of cleaved-PARP, cleaved-caspase-3, Cyto-c and BAD. (F) Histogram shows the relative abundance of apoptosis-related proteins compared with the control group. The data shown are the mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, and ns, nonsignificant). n = 3 biological replicate samples.