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Fig. 2 In the absence of Zbtb11, FL-committed progenitor numbers fail to increase. (A) Schematic description of the analysis. Flow cytometry analysis dot plots show representative incidence at E17.5: (B/B′) LMPP (LSKCD34+Flt3+); (D/D′) CMP (Lin−CD127−Sca-1−c-Kit+CD34+CD16/32−), GMP (Lin−CD127−Sca-1−c-Kit+CD34+CD16/32+), and MEP (Lin−CD127−Sca-1−c-Kit+CD34−CD16/32−); and (H/H′) CLP (Lin−CD127+Sca-1+cKit+Flt3+). Scatter plots showing the absolute quantification of total content at E14.5, E16.5, and E17.5: (C) LMPP, (E) CMP, (F) GMP, (G) MEP, and (I) CLP. For panels C and I: E14.5 WT, n = 22 and KO n = 7; E16.5 WT, n = 16 and KO n = 4; E17.5 WT, n = 8 and KO n = 2. For panels E-G: E14.5 WT, n = 22 and KO, n = 7; E16.5 WT, n = 16 and KO, n = 4; E17.5 WT, n = 16 and KO, n = 5. WT/controls (green); KO, Zbtb11hKO (red); data ± standard error of the mean. Two-way analysis of variance with Tukey (black) or Šídák (blue) multiple comparisons: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. CLP, common lymphoid progenitor; GMP, granulocyte–macrophage progenitor; LMPP, lymphoid-primed multipotent progenitor; MEP, myeloerythroid progenitor.