|
Fig. 1 Dhx38 deletion impaired retinal morphology and RPC differentiation (A) The morphology of bodies and eyes in siblings and dhx38−/− embryos at 24, 30, 36, and 48 hpf. Scale bar, 100 μm. (B, C) Quantification of the embryonic length and eye size in siblings and dhx38−/− embryos shown in A. n = 10 for each panel. Data was shown as mean ± SD. n.s., no significance; ∗∗p < 0.01, ∗∗∗∗p < 0.0001 as indicated. (D) Retinal sections of siblings and dhx38 mutants were stained with phalloidin and DAPI at 36 and 48 hpf. V, ventral side, D, dorsal side. n = 9 for each panel. Scale bar, 50 μm. (E) Whole-mount in situ hybridization for RPCs marker (ccnd1 and vsx2), for neural precursors (atoh7 and crx1), and for specialized neurons (neurod1) and mature neurons (tuba1) at 36, 48 and 60 hpf. Scale bar, 100 μm. (F) Retinal sections of siblings and dhx38−/− embryos were immunostained using Sox2 (a marker for RPCs), and Islet1 (a marker for neuron cells) antibodies at 48 hpf. (G and H) Quantification of the Sox2+ and Islet1+ cells in the retinas of siblings and dhx38−/− embryos shown in F. n = 6 per panel. Scale bar, 50 μm. Data was shown as mean ± SD. ∗∗p < 0.01, ∗∗∗∗p < 0.0001 as indicated. (I) Distribution of Neurod1:EGFP (specialized neurons) and Huc:EGFP (post mitotic neurons) labeled cells in the whole retina of siblings and dhx38−/− transgenic zebrafish at 36, 48 and 60 hpf. The dashed circles indicate the eye and lens, respectively. Scale bar, 50 μm. (J) Quantification of the GFP intensity in the eyes of siblings and dhx38−/− embryos at different stages shown in I. n = 10 per panel. Data was shown as mean ± SD. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 as indicated.