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Fig. 4 Effect of neutrophil activity–specific chemicals on liver pathogenesis in atp7b-/- zebrafish. (A) Representative H&E staining images of liver sections from wild-type and mutant fish in response to LPS, FPR A14, or PR 39. Scale bar: 200 μm. qPCR of gene expression changes in the lipogenic factors (B) pparα, (C) pparγ, (D) srebp1, and (E) ucp2, and neutrophil-specific factors (F) lyz and (G) mpx in both wild-type and mutant fish livers response to LPS, FPR A14, or PR 39. Data are the means ± SEM, n = 3 sets of 20 fish/group. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001. (H) Inductively coupled plasma mass spectrometry of liver Cu content in wild-type and mutant fish in response to LPS, FPR A14, or PR 39. Data represent the means ± SEM, n = 3 sets of 1000 fish/group. ∗∗P < .01.