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Fig. 1 Neutrophils infiltrate into livers in atp7b-/- zebrafish indicated by lyz-positive neutrophils. (A) Representative images of lyz+/atp7b+/+ fish and lyz+/atp7b-/- fish after 0, 6, 12, 18, and 24 hours of Cu treatment. The livers are outlined in blue dashed lines. hpt, hours post-treatment. Scale bar: 200 μm. Liver (B) neutrophil count, (C) size, and (D) neutrophil density (liver neutrophil density = liver neutrophil count/liver size) in wild-type and mutant fish. Data are the means ± SEM, n = 10 fish/group. ∗∗P < .01.
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