Figure 2

Figure 2

The liver visual phenotype of zebrafish larvae at 176 h post-fertilization. (A) Oil Red O staining was utilized to observe lipid droplets in the entire body and liver of zebrafish larvae. (B) Liver Oil Red O staining was quantitatively analyzed using ImageJ software (version 1.53), with results expressed as a percentage relative to the M group (mean ± SD; n = 3). (C) The inhibition rate of liver steatosis was determined by Oil Red O staining. (D) The impact of LJP on the cardiac rate of zebrafish larvae was evaluated. # p < 0.05, ## p < 0.01, and ### p < 0.001 represent significant differences compared to the M group using the unpaired 2-tailed Student’s t-test. Different lowercase letters indicate significant differences between groups according to the One-way ANOVA followed by post hoc Duncan’s for multiple comparisons. (E) Histological images of zebrafish livers from control (a), model (b), A_0.05 (c), and LJP_0.20 (d) groups. (F) Nile red staining, coupled with nuclear DNA staining, revealed lipid droplets in the liver cells of zebrafish larvae.