Fig. 5.

Fig. 5.

The cardiac transcriptomes of Tnnt2a degrons and tnnt2a mutants display high similarity at the single cell level. (A) Schematic and description of the sample preparation for single-cell RNA-sequencing; 100 hearts from 72 hpf WT, tnnt2a^{mn0031Gt/mn0031Gt} (Mutant), and tnnt2a^{mn0031Gt/bns513}; myl7:zGRAD-P2A-TagBFP^+/− (Degron) larvae were dissected, dissociated and the single cell suspension sequenced. (B) Uniform manifold approximation and projection (UMAP) representation of the data; stacked columns represent the numbers of WT (green), mutant (orange), and degron (violet) cells for each annotated population; UMAP is displayed merged (Left) or split by genotype (Right).