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Fig 2
SsuCC20p is phase variable, and three alleles can be found within a single isolate. (A) Graphical representation of the hsdS allele quantification by FAM-labeled PCR product endonuclease digestion and fragment analysis. (B) Representative example of WT 861160 hsdS allele identification; red peaks are the LIZ1200 size marker, and blue peaks, the FAM-labeled fragments. (C) hsdS allele distribution was computed by measuring the relative area under the curve of the different hsdS alleles using PeakScanner v3.0 in three biological replicates. (D) hsdS alleles were quantified using PacBio or (E) Oxford Nanopore long-read sequencing data; read count per allele is indicated above each bar. N.D., not detected; AU, arbitrary units.