Fig. 3

Fig. 3 Leydig cells in Trpv4 KOs did not undergo apoptosis in high temperature.

a HE staining of testes. The upper panels show Trpv4^+/+ and the lower panels show Trpv4^−/−. Black line indicates areas of E3 spermatid. Red line indicates an abnormal cell population. Arrows indicate interstitial tissue. Interstitial tissue can be detected clearly in Trpv4^−/− after 7 and 14 days of temperature stimulation. b qRT-PCR analysis of Leydig cell marker genes in testes. Values are the mean ± SEM (n = 4). *p < 0.01 compared with the 29 °C testis. c In situ hybridization in testes of Isl3, which is a Leydig cell marker. d In situ hybridization of Hspa5. Black broken lines indicate interstitial cells and red broken lines indicate spermatocyte cells. e Localization of cleaved caspase 3. White broken lines indicate interstitial cells and red broken lines indicate spermatocyte cells. Scale bars are 20 μm.