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Fig. 6 Microbiota-promoted HSPC development is dependent on basal inflammatory cytokine expression (A) WISH for cmyb and mpx in control and il1b morphant larvae. (B) Quantification of WISH results from (A). (C) WISH for cmyb and mpx in control and tnfa morphant larvae. (D) Quantification of WISH results from (C). (E) WISH for cmyb and mpx in WT CONR, GF larvae, and GF larvae at 3 dpf treated with microbiota from the CONR WT (CONV) with or without MCC950. (F) Quantification of WISH results from (E). (G) WISH for cmyb in WT and myd88−/− CONR, GF larvae, and GF larvae at 3 dpf treated with microbiota from the CONR WT (CONV). (H) Quantification of WISH results from (G). Student’s t tests in (B and D), ANOVA in (F and H); means ± SEM of three independent experiments, n = 45–60 larvae per group for WISH, ∗p < 0.05, ∗∗p < 0.01; ns, not significant. Scale bars, 200 μm. See also Figure S7.