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Fig. 3 Dysregulated microbiota in chd8−/− inhibits HSPC development and enhances myeloid differentiation (A) WISH for cmyb and mpx in 5-dpf WT and chd8−/− CONR and GF larvae, and in WT and chd8−/− GF larvae at 3 dpf conventionalized with microbiota from the CONR WT (CONV). (B and C) Quantification of WISH results from (A). (D) qPCR analysis of runx1 and cmyb expression in the kidneys of WT CONR, chd8−/− CONR, GF, and CONV larvae at 7 dpf. (E) qPCR analysis of mpx expression in the whole body of WT CONR, chd8−/− CONR, GF, and CONV zebrafish at 7 dpf. (F) WISH for cmyb and mpx in 5-dpf WT CONR, GF larvae, and GF larvae at 3 dpf conventionalized with WT-sourced microbiota (M-W) or chd8−/−-sourced microbiota (M-C) obtained from adult intestines. (G) Quantification of WISH results from (F). (H) qPCR analysis of runx1 and cmyb expression in the kidneys at 7 dpf of WT CONR, GF, and GF larvae at 3 dpf conventionalized with M-W or M-C. (I) The mpx expression in the whole body at 7 dpf of WT CONR, GF, and GF larvae at 3 dpf conventionalized with M-W or M-C. ANOVA in all analyses; mean ± SEM of three independent experiments, n = 45–60 larvae per group for WISH, 30–40 larvae per group for qPCR analysis, ∗p < 0.05, ∗∗p < 0.01, ns, not significant. Scale bars, 200 μm.