Figure 2

Figure 2

Cartilage staining with alcian blue of 5 dpf (A,C) and 10 dpf (B,D) wt and efemp1−/− mutant larvae. The different measures are illustrated in (A,B): d-ar: distance between the Meckel’s-palatoquadrate articulations; d-cer: distance between the posterior ends of the ceratohyals; a-cer: angle between the ceratohyals; hl: head length and d-hyo: head width. (C) efemp1−/− reveal a reduced distance between the articulation (d-ar) and narrower distance between the ceratohyal elements (d-cer) at 5 dpf compared to wt (wt n = 24, efemp1−/− n =21). (D) No difference in the cartilage elements in efemp1−/− at 10dpf compared to wt (wt n = 22, efemp1−/− n = 27). The graphs show the individual data points, the mean value ± SEM; significance: * p < 0.05 (unpaired student’s t-test).