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Fig. S4 The conditional knockin of nkx2.5 partially rescued ventricular chamber maturation. A: The WISH results show the reduced expression of nkx2.5 in foxc1a nju18 hearts. Sample size: WT (n = 10), foxc1a nju18 (n = 15) at 60 hpf; WT (n = 9), foxc1a nju18 (n = 5) at 72 hpf; WT (n = 14), foxc1a nju18 (n = 9) at 96 hpf. B: The qRT-PCR results show the reduced expression of nkx2.5 in foxc1a nju18 hearts at 72 and 96 hpf. C: Mid-sagittal confocal images show the representative hearts of 96 hpf WT (n = 7) and foxc1a nju81 (n = 10) embryos. D: The statistical analysis derived from C exhibits the rescued trabeculae structure in foxc1a nju81 hearts. E: 96 hpf cryosections were stained with WGA to observe cell-membranes. F: A scatter plot shows the decreased heart rate in 108 hpf foxc1a nju81 hearts. Sample size: WT (n = 19), foxc1a nju81/+ (n = 29), foxc1a nju81 (n = 33). Scale bar, 50 μm. ns, P > 0.05; ***, P < 0.001. Error bars, S.D.
Reprinted from Journal of genetics and genomics = Yi chuan xue bao, 49(6), He, L., Zhang, Q., Jiang, D., Zhang, Y., Wei, Y., Yang, Y., Li, N., Wang, S., Yue, Y., Zhao, Q., Zebrafish foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression, 559-568, Copyright (2021) with permission from Elsevier. Full text @ J. Genet. Genomics