ZFIN Image: Tian et al., 2024, Fig. 2

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Figure Caption

Fig. 2

Late-stage adipogenic genes express earlier abnormally. a Illustration of the early stage and late stage during adipogenesis. b–e qRT-PCR analysis of early circadian genes PER1 (b), ANDPTL4 (c), ACAT2 (d), and CHST7 (e) in hMSCs (N = 3 biological replicates). f–i qRT-PCR analysis of late circadian genes RORB (f), ME 1 (g), CYP1B1 (h), and LAMA 2 (i) in hMSCs (N = 3 biological replicates). j, k Representative immunostaining images of PER1 (j) and RORB (k). l, m qRT-PCR analysis of PER1 (l) and RORB (m) in hESCs-derived white adipocytes (N = 3 biological replicates). n, o qRT-PCR analysis of PER1 (l) and RORB (m) in hESCs-derived brown adipocytes (N = 3 biological replicates). Data are presented as the mean ± S.D. Statistical analysis was performed using two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001

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