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Fig. 6 NRT attenuates endoplasmic reticulum (ER) stress and mitochondria function in zebrafish larvae with alcoholic injury. (A) mRNA expression of genes associated with ER stress upstream regulator (atf4, atf6, ire1), pro-survival (xbp1-unsplicing, xbp1-splicing), molecular chaperon (bip, dnajc3, grp94) and cell death (ddit3, edem1, gadd45a). (B) Changes of genes associated with mitochondrial injury (bim, bida, baxb) in EtOH-treated zebrafish larvae by NRT (n = 15/group, three independent experiments). (C) Protein expression levels of Ddit3, Bip and Gapdh. Quantification of band intensity of Ddit3 and Bip was calculated by Gadph as a housekeeping protein (n = 5 biologically independent experiments). The data are presented as the mean ± SD; a,b,c,d mean values with different superscript letters are significantly different among the groups (p < 0.05).