Regulation of epithelial–mesenchymal transition (EMT) in NSCLCs by MP06. (A) Influence MP06 on NSCLCs migration (24 h) and invasion (48 h). Scale bars: 200 µm. (B) Expression level of epithelial–mesenchymal transition (EMT) markers with 10 μM MP06 compared water-treated control cells (contr) in NSCLCs for 48 h using RT-PCR and Western blotting assay. GAPDH and β-actin were used as housekeeping genes on the basis of their consistency of expression. (C) The expression of vimentin in A549 and H460 cells treated with MP06 using Immunofluorescence (IF) analysis. Scale bars: 100 µm. ** p < 0.01 and *** p < 0.001.
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