ZFIN Image: Zhai et al., 2023, Figure 6

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Figure 6

Expression and purification of recombinant zebrafish IL-6 (rDrIL-6) protein. (A) Analysis of rDrIL-6 protein by SDS-PAGE and WB. The utilized expression vector was pET-32a. The expression of the recombinant zebrafish IL-6 (rDrIL-6) protein was induced at 37 °C for 4 h with different concentrations of IPTG (1.0 and 1.5 mM). KDa: kilo Dalton; M: marker; 1: control group; 2: 1.0 mmol/L IPTG-induced group; 3: 1.5 mmol/L IPTG-induced group; (B) 4: assessment of purified rDrIL-6 protein by SDS-PAGE; (C) 5: confirmation of purified rDrIL-6 protein by WB. Anti-His antibody was used as a primary antibody.

Acknowledgments

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