ZFIN Image: Zhai et al., 2023, Figure 1

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Figure Caption

Figure 1

Generation of il-6^−/− zebrafish via CRISPR/Cas9 technology. (A) Diagram of the IL-6 single-guide RNA (sgRNA). Boxes depict exons while lines depict introns. Protospacer adjacent motif (PAM) and target sequences are indicated in blue and red, respectively. (B) Sequencing results of wild-type (WT) and il-6 knockout homozygous (il-6^{−/−−/−}) zebrafish. The red frame indicates base deletion. According to the DNA sequencing results, il-6^−/− zebrafish with 7 bp deletion mutation were obtained. (C) Protein domains of the il-6 gene in WT and il-6^−/− zebrafish. The TMD domain is shown in blue, the IL6 domain in green, and the mutated region of the protein in yellow. il-6^−/− zebrafish showed a frameshift mutation in IL-6, resulting in premature termination of translation compared to wild-type zebrafish.

Acknowledgments

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