Fig 3

Fig 3

DSS causes intestinal epithelial damage and inflammation consistent with enterocolitis. (A) Representative hematoxylin- and eosin-stained longitudinal sections (n = 4) of the anterior, mid, and posterior intestine from untreated (Ai–iii) and DSS-treated (Aiv–vi) larvae at 6 dpf; scale bars = 50 µm. (B) Representative confocal images of live UT (i, iii) and DSS-treated (ii, iv) Tg(mpo::egfp) larvae at 6 (i-ii) and 7 (iii-iv) dpf; neutrophils (green); larvae were imaged for 18 hours (3–20 hpi). Scale bars = 200 µm. (C) Quantification of neutrophils in the intestine at 6 and 7 dpf (3 and 4 days post DSS treatment); unpaired two-tailed t-test, n ≥ 11. (D) qRT-PCR analyses of cxcl8, il1b, mmp9, and tnfa in DSS-treated larvae relative to untreated controls at 6 dpf and (E) 7 dpf; n = 3. Unpaired two-tailed t-test. Mean ± SEM, *, P ≤ 0.05; **, P ≤ 0.01; ns, not significant.