Fig 2

Fig 2

Larval zebrafish treated with 0.5% DSS have decreased survival and intestinal growth rates. (A) Schematic outlining timeline of DSS administration (red, 3–6 dpf) and survival experiments (blue, 1–7 days post exposure). (B) Survival of larvae administered 0.5% DSS (black circles) relative to untreated (UT) controls (empty circles). Data were analyzed using a Kaplan-Meier plot and Mantel-Cox test; ****, P ≤ 0.0001, n = 20. (C) Quantification of swim bladder defects in UT or DSS-treated larvae. Group differences were analyzed using Mantel-Cox test; ****, P ≤ 0.0001, n = 20. (D) Gut-to-body length ratio was analyzed by linear regression; ns = not statistically significant. Data are means ± SEM from n = 20. (E) Representative images of untreated (Ei) and DSS-treated (Eii) larvae at 6 dpf (3 days post DSS exposure), with the swim bladder (teal) and the intestine (red) outlined. Scale bar = 0.3 mm.