Figure 5

Figure 5

Disruption of phd3 in zebrafish facilitates hypoxia tolerance.A, photographs of phd3-null zebrafish larvae and their wildtype siblings (phd3^−/− or phd3^+/+) (3 dpf) under normoxia (21% O₂) or hypoxia (2% O₂) for 15 h. Red arrows indicate dead zebrafish. B, survival curve of phd3-null zebrafish larvae and their wildtype siblings (phd3^−/− or phd3^+/+) (3 dpf) under hypoxia (2% O₂). C, photographs of phd3-null adult zebrafish and their wild-type siblings (phd3^−/− or phd3^+/+) (4 mpf) under hypoxia (5% O₂) for 0 h, 2 h, and 4 h. Red arrows indicate dead zebrafish. D, survival curve of phd3-null adult zebrafish and their wildtype siblings (phd3^−/− or phd3^+/+) (4 mpf) under hypoxia (5% O₂). ns, not significant; ∗p < 0.05, ∗∗∗∗p < 0.0001, using log-rank test analysis; data are representative of three independent experiments.