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Fig. 3 zPRDM14, but not sPRDM14, maintains the self-renewal of mPrdm14 KO ESCs under the serum plus LIF condition. (A) The growth rate of mPrdm14 KO ESCs expressing the empty vector, sPrdm14, zPrdm14 or mPrdm14 under the serum plus or 2i plus LIF condition. The mean values are shown with the s.e.m. (three biological replicates). (B) Relative expression levels of the indicated genes. Each Ct value measured under the 2i plus LIF condition was subtracted from that measured under the serum plus LIF and is shown with the s.e.m. (two technical replicates). (C,D) AP activity of mPrdm14 KO ESCs expressing the empty vector, sPrdm14, zPrdm14 or mPrdm14 cultured in medium containing serum plus LIF for 9 days (C) or 2 weeks at seven passages (D). Scale bars: 50 μm. (E) Relative expression levels of the indicated genes in wild-type ESCs and mPrdm14 KO ESCs expressing zPrdm14 or mPrdm14 and cultured for 2 weeks. Each Ct value was subtracted from that in wild-type ESCs and is shown with the s.e.m. (two technical replicates).