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Fig. 2 zPRDM14, but not sPRDM14, compensate for transcriptional regulation in mPrdm14 KO ESCs. (A) Western blot analysis of PRDM14 orthologues. (B) Morphology of wild-type ESCs and mPrdm14 KO ESCs expressing the empty vector, sPrdm14, zPrdm14 or mPrdm14. Scale bar: 50 μm. (C) Relative expression levels of the indicated genes in wild-type ESCs and mPrdm14 KO ESCs expressing the empty vector, sPrdm14, zPrdm14 or mPrdm14. Each Ct value was subtracted from the Ct values for wild-type ESCs and is shown as the log2 with the s.e.m. (two technical replicates). (D) ChIP-qPCR analysis for the enrichment of the indicated PRDM14 orthologues in mPrdm14 KO ESCs. The enrichment of each orthologue is indicated by the percent input with the SEM (two technical replicates). nd, not detected.