Fig. 5

Fig. 5

Genetic inhibition of ANXA2 prevents tissue colonization of MDA-MB-231 cells in NOD SCID mice. A Control or shANXA2 MDA-MB-231 cells were injected in NOD SCID mice as described in the legend of the Supplementary Fig. S10. Representative bioluminescence images of NOD SCID mice 30 min (D0) or 70 days (D70) after injection of MDA-MB-231 cells are presented. B Bioluminescence quantification in lungs and bone for control (n = 5) or shANXA2 (n = 5) MDA-MB-231 cells. The black arrow points the time when some mice (n = 4), initially injected with shANXA2 MDA-MB-231 cells and exhibiting low signal in bioluminescence imaging, were injected with control (n = 2) or ANXA2 shRNA (n = 2) MDA-MB-231 cells. C Representative bioluminescence images of NOD SCID mice, initially injected with shANXA2 MDA-MB-231 cells and, injected a second time with either control or shANXA2 MDA-MB-231 cells, at 30 min (D0) or 76 days (D76) after the second injection. D Bioluminescence quantification in lungs and bone for control (n = 2) or shANXA2 (n = 2) MDA-MB-231 cells after the second injection