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Fig. 3 Csf1rb deficiency impairs the proliferation capacity of HSPCs. (A) Immunostaining of cmyb-GFP (green) and BrdU (red) in the CHT at 3 dpf in siblings and csf1rb mutants after BrdU labeling for 1 h. Yellow arrows show BrdU+ HSPCs, white arrows indicate GFP single-positive HSPCs. Scale bars: 20 μm. (B) Quantification of cmyb-GFP+ HSPCs in the CHT at 3 dpf in siblings (n=16) and csf1rb mutants (n=19). (C) Quantification of the proliferation ratio of cmyb-GFP+ HSPCs in the CHT at 3 dpf in siblings (n=16) and csf1rb mutants (n=19). Data are presented as mean±s.e.m. **P<0.01.
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