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Fig. 6 Semaphorin 3 ligands regulate trunk lymphatic guidance. (A-F) Confocal images of the head (A-C) or trunk (D-F) vasculature showing the blood (green) and lyve1b-expressing vessels (white) in 6 dpf lyve1b:DsRed; fli1a:EGFP wild type (A,D) and sema3aasa10241 (B,E) or sema3csa15161 (C,F) mutants. Yellow arrowheads indicate misaligned ISLVs. (G) Quantitation of misaligned ISLVs at 6 dpf. (H) Quantitation of aberrant facial lymphatics at 6 dpf. (I) Quantitation of facial LEC number at 6 dpf. P>0.05 (not significant), **P<0.01 (by Mann–Whitney test); data are mean±s.d. Scale bar: 50 µm. ISLV, intersegmental lymphatic vessel. Numbers in graphs represent numbers of larvae.