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Fig. 5 Aerobic exercise downregulates miR-128 and activates insulin-like growth factor-1 signaling in aging skeletal muscle. (A) Effect of different concentrations of d-galactose with aerobic exercise on miR-128 expression in zebrafish skeletal muscle. (B) An analysis of the human and zebrafish insulin-like growth factor-1 (IGF-1) sequence for a potential miR-128 binding site. The blue and red sections indicate the seed region of miR-128 and the binding site of the seed region to igf1 3?UTR, respectively. (C) Quantitative analysis of the dual-luciferase reporter assay. IGF-1-WT: synthesized wild-type IGF-1 3?UTR pmirGLO vector that contains the miR-128 predicted binding site; IGF-1-MUT: synthesized mutated IGF-1 3?UTR pmirGLO vector that contains the mutated miR-128 predicted binding site; miR-NC: negative control miRNA. * represents d-gal-treated controls (DC) compared with normal controls (NC); # represents d-gal aerobic exercise (DA) compared with DC. #, * p < 0.05. ## p < 0.01. ###, *** p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)