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Fig. 2 Aerobic exercise counteracts the d-galactose-induced reductions in skeletal muscle function and mass. (A) Changes in the body size of zebrafish in each group after treatment. (B) Body weights of zebrafish in each group at the end of the 4-week treatment period. (C, D) Effects of aerobic exercise on the critical swimming speed (Ucrit) and exercise oxygen consumption (AMR) in D-galactose (d-gal)-induced aging zebrafish. (E) Hematoxylin and eosin staining of skeletal muscles in each group of zebrafish. Scale bar: 500 ?m. (F) The cross-sectional area of skeletal muscle (?m2) in each zebrafish group. (G) Masson's trichome staining of zebrafish skeletal muscle tissue in each group. Blue and red show collagen fiber and myofiber staining, respectively. Scale bar: 100 ?m. (H) Fibrosis fraction (%) of each zebrafish group. * represents d-gal-treated controls (DC) compared with normal controls (NC); # represents d-gal aerobic exercise (DA) compared with DC. #, * p < 0.05. ###, *** p < 0.001. ns, not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)