ZFIN Image: Forti et al., 2023, Fig 4

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Figure Caption

Fig 4

Phage-resistant mutants have attenuated virulence in zebrafish. Left panel. Survival of zebrafish embryos (N = 60) infected with the indicated P. aeruginosa strains. Kaplan-Meyer curves represent results deriving from two independent experiments in which groups of 30 embryos were injected with the indicated strains. hpi, hours post-infection. Significance of the difference with respect to survival upon PAO1 infection was estimated with the Gehan-Breslow-Wilcoxon test. ***P < 0.001. ns, not significant. Right panel, bacterial burden estimated as described on four groups of five embryos for each condition. Significance of the difference with respect to the average bacterial burden in PAO1 set to 1 (dashed line) was estimated with t-test. ***P < 0.001, and **P < 0.01; ns, not significant. wapH⁻, PAER5b; galU⁻, PAER6b; wzy⁻, PADR6; algC⁻, PAER10b; ΔpilQ, PAMO302.

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Acknowledgments

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