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Figure 1.

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Publication: Singh et al., 2023 - NMJ analyser: a novel method to quantify neuromuscular junction morphology in zebrafish
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Figure 1.

Manual analysis of NMJ morphology is time-consuming and prone to bias. Confocal imaging of zebrafish NMJs, stained with presynaptic marker SV2a and postsynaptic marker α-bungarotoxin (α-BTX), entails acquisition of 80–100 z-slices (N). Each z-slice contains an estimated 50–100 synaptic puncta (n). The manual quantification of individual puncta within separate channels (2) and the assessment of their co-localization necessitate a substantial investment of time and effort, particularly when analyzing multiple samples representing diverse experimental conditions and varying time points. Moreover, limitations in the resolution of confocal microscopy introduce the possibility of under or overestimation of synaptic puncta counts.

Acknowledgments

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