IMAGE
Figure 2.
- ID
- ZDB-IMAGE-231212-28
- Publication
- Singh et al., 2023 - NMJ analyser: a novel method to quantify neuromuscular junction morphology in zebrafish
- All Figures
- Figures for Singh et al., 2023
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Figure Caption
Figure 2.
NMJ analysis workflow overview (Steps 1–7). Z-projection are created of the confocal images where users are prompted to define the ROIs. Each z-slice is processed for contrast enhancement and punctum detection via Otsu thresholding. Clustered puncta are segmented into single punctum and co-localization analysis is performed for each punctum in the defined ROI.
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