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Fig. 6 Inhibition of the Notch signaling pathway leads to an increase in tph2+ neurons (A and B) HCR RNA-FISH against tph2 mRNA. Arrowheads indicate tph2-expressing neurons. (A) Control embryo treated with DMSO. (B) Embryo treated with the Notch inhibitor LY. (C) Quantification of tph2+ neurons. Embryos were treated from 2 to 3 dpf. The dorsal side is oriented upwards, and the anterior side is oriented toward the left. Statistical significance was assessed using Welch’s t-test, and the level of significance is indicated by the number of asterisks: ∗∗ for p < 0.01. Scale bar: 20 μm.
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