ZFIN Image: Landi et al., 2023, Fig. 4

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Figure Caption

Fig. 4 Effect of overexpression of WT, p.Ala630Pro, p.Gln474Arg and p.Lys632Asn STAT5B mRNA on zebrafish embryos. (A, C, E) Representative pictures of zebrafish embryos with pericardial edema (A), cyclopia (B) and bent spine (E) during development. (B, D, F) Statistical analysis of pericardial edema score (B), cyclopia (D) and bent spine (F) in embryos overexpressing WT, p.Ala630Pro, p.Gln474Arg and p.Lys632Asn STAT5B mRNA. (G) Percentage of embryos showing pericardial edema, cyclopia, and bent spine in embryos overexpressing WT, p.Ala630Pro, p.Gln474Arg and p.Lys632Asn STAT5B mRNA. Embryos at 72 hpf were classified into three groups according to the severity of the malformation and scored (1 = normal, 2 = mild and 3 = severe). Mann–Whitney test, ***P < 0.0001, *P < 0.05. N = number of embryos across three independent biological replicates.

Acknowledgments

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