ZFIN Image: Cai et al., 2023, Figure 1

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Figure Caption

Figure 1

Schematic diagrams of asb5a gene knockout in zebrafish. (A) Schematic diagram of sgRNA targeting for asb5a gene knockout. Green horizontal line represents the genomic DNA of asb5a, green rectangle represents the exons of asb5a, red font represents the target site sequence, and blue font represents the protospacer adjacent motif (PAM). (B) Schematic diagram of the three heritable mutant alleles of asb5a produced via gene knockout and their encoded protein sequences. (C) Sequencing peak diagram of three asb5a mutant alleles, in which bases A, G, C, and T are represented by green, black, blue, and red curves, respectively.

Acknowledgments

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