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Fig. 2 TSAR.0878-chicken expression analysis in the hearing and lateral line systems in transgenic zebrafish. (A) At the top: schematic of the transgene containing the TSAR.0878-chicken sequence cloned upstream of the cFos murine minimal promoter and the reporter gene eGFP. Below: detail of the middle part of the TSAR.0878 sequence alignment including the tested sequences (mouse and chicken) and other mammal representative sequences. (B–M) Fluorescent microphotographs of one representative transgenic zebrafish line at 7 days post fertilization (dpf) carrying the TSAR.0878-chicken transgene showing eGFP expression (B, E, H, and K), the hair cell-specific marker FM4-64 (C, F, I, and L), and the overlay (D, G, J, and M). The expression of eGFP coincides with the hair cell marker in neuromasts of the lateral line and in the otic capsule (E, G, and F) of the zebrafish. (F) shows a magnification of the otic capsule region. (N, O, and P) show PKNOX2 expression by in situ hybridization at 7 dpf. Arrows indicate the location of neuromasts of the hearing and balance systems expressing PKNOX2. Ba, branchial arches.